Journal: Toxicon: X

Article Title: Biochemical characterisation and substrate-specific proteolytic diversity of venom metalloproteinases in African puff adders

doi: 10.1016/j.toxcx.2026.100253

Figure Lengend Snippet: Insulin B degradation assay: Purif ied SVMPs. SVMPs were incubated at 37 °C for 90 min with oxidised insulin B chain at a ratio of 30:1 (w/w) insulin B: SVMP. An aliquot containing the equivalent of 1 μg insulin B was analysed by RP-HPLC with monitoring at 214 nm. The conditions for each are indicated in the inset. The result of an EDTA treated SVMP is shown for just one of the SVMPs (TZA SVMPI): all others were identical.

Article Snippet: An aliquot containing the equivalent of 1 μg insulin B was analysed by RP-HPLC using a Biobasic C4 column (2.1 × 150 mm).

Techniques: Degradation Assay, Incubation

Journal: Toxicon: X

Article Title: Biochemical characterisation and substrate-specific proteolytic diversity of venom metalloproteinases in African puff adders

doi: 10.1016/j.toxcx.2026.100253

Figure Lengend Snippet: Conversion of angiotensin I to angiotensin 1-7 by the 21 kDa TZA SVMP. SVMPs were incubated at 37 °C for 90 min with angiotensin I at a ratio of 30:1 (w/w) angiotensin I: SVMP. An aliquot containing the equivalent of 1 μg angiotensin I was analysed by RP-HPLC with UV monitoring at 214 nm. The mass values for the products shown next to the relevant peaks were obtained from concomitant RP-HPLC-MS analysis carried out on the same reaction mix.

Article Snippet: An aliquot containing the equivalent of 1 μg insulin B was analysed by RP-HPLC using a Biobasic C4 column (2.1 × 150 mm).

Techniques: Incubation